The world of RNA research is evolving rapidly, driven by advancements in RNA therapeutics, particularly in the realm of circular RNA (circRNA). As a groundbreaking method, circular RNA offers robust stability, making it a promising candidate for therapeutic applications. At IntegrateRNA, we provide top-tier circular RNA synthesis services designed to cater to diverse research requirements.

The Potential of Circular RNA

As RNA vaccines continue to gain momentum, one of the significant challenges faced in the field has been ensuring the stability of RNA molecules. Circular RNAs present an advantageous alternative. Their unique structure contributes to enhanced stability, making them more resistant to degradation from exonucleases. Studies indicate that circular RNAs are approximately two to five times more stable than their linear counterparts, leading to increased interest in their potential as therapeutic agents.

Our Expertise in Circular RNA Synthesis

IntegrateRNA has dedicated years to refining the circular RNA synthesis process, establishing advanced platforms tailored for RNA drug discovery. Our synthesis capabilities leverage both the T4 DNA/RNA ligase circularization method and our proprietary CB^ITR circularization system. This dual approach enables us to produce customized circular RNA products in both research-grade and pharmaceutical-grade formats. Researchers can simply submit their target RNA sequences for an in-depth consultation and project assessment.

Key Features of Our Circular RNA Synthesis:

• CB^ITR Circularization Method:

  • Absence of exogenous sequence residues
  • High ligation efficiency exceeding 90%
  • Simple reaction and purification conditions
  • Customizable RNA lengths ranging from 20 to 300 nucleotides
  • Quality options: research-grade (85-90% purity) and pharmaceutical-grade (over 95% purity)

• Enzymatic Method Highlights:

  • High efficiency for small circular RNAs
  • No residual exogenous sequences

• Ribozyme Method Advantages:

  • Simple purification processes
  • High circularization efficiency

Workflow of Circular RNA Synthesis

1. Sequence Evaluation and Strategy Design: Tailoring the circularization strategy based on sequence.
2. Template Preparation and In Vitro Transcription: Synthesizing linear RNA.
3. RNA Purification: Ensuring purity before circularization.
4. RNA Circularization: Applying the desired circularization method.
5. Quality Control: Conducting RNase R digestion, junction validation, and RNA integrity analysis.
6. Final Purification: Using HPLC and LiCl precipitation.
7. Delivery: Product is freeze-dried and shipped to the customer.

IntegrateRNA’s circular RNA synthesis service is crafted to meet a wide range of research needs with a commitment to quality, efficiency, and reliability. Whether you need fast turnaround times or competitive pricing, our team is ready to assist you in achieving your research objectives.

Support and Consultation

For any inquiries regarding our circular RNA synthesis service or if you require additional information, please do not hesitate to contact us. Our customer service team is available to provide the necessary support throughout your research journey.